Description
Description: This assay employs a two-site sandwich ELISA to quantitate CBLN3 in samples. An antibody specific for CBLN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CBLN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CBLN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CBLN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Members of the precerebellin family, such as CBLN3, contain a cerebellin motif and a C-terminal C1q signature domain that mediates trimeric assembly of atypical collagen complexes. However, precerebellins do not contain a collagen motif, suggesting that they are not conventional components of the extracellular matrix. The cerebellin motif of Cbln3 is quite different from that of Cbln1, indicating that Cbln3 is not a cerebellin precursor. Northern blot analysis detected several Cbln1 transcripts in adult mouse cerebellum, but not in forebrain, spinal cord, or any other adult mouse tissue examined. Examination of brain samples from embryonic and young mice revealed that Cbln3 was not expressed until postnatal day 7. In situ hybridization of adult brain showed Cbln3 in the internal granule cell layer of the cerebellum and in the dorsal cochlear nucleus